Automated Multiscale Morphometry of Muscle Disease From Second Harmonic Generation Microscopy Using Tensor-Based Image Processing
نویسندگان
چکیده
منابع مشابه
Interferometric second harmonic generation microscopy.
We describe a novel second harmonic generation (SHG) microscope that employs heterodyne detection by interfering the epi directed SHG from a sample with SHG from a reference crystal. In addition, the microscope provides complementary reflectance information based on optical coherence microscopy (OCM). The instrument features dual balanced detection to minimize the effect of source fluctuations,...
متن کاملAutomated biphasic morphological assessment of hepatitis B-related liver fibrosis using second harmonic generation microscopy
Liver fibrosis assessment by biopsy and conventional staining scores is based on histopathological criteria. Variations in sample preparation and the use of semi-quantitative histopathological methods commonly result in discrepancies between medical centers. Thus, minor changes in liver fibrosis might be overlooked in multi-center clinical trials, leading to statistically non-significant data. ...
متن کاملTwo Photon Microscopy and Second Harmonic Generation
Nonlinear microscopy has become an important tool for imaging biological samples such as neurons. In two photon fluorescence, a fluorophore absorbs two photons at a low energy state, one of its electrons briefly converts to a higher energy state, and it emits a single excited photon at an energy level higher than that of the absorbed photons. In second harmonic generation (SHG), laser light is ...
متن کاملImaging skeletal muscle using second harmonic generation and coherent anti-Stokes Raman scattering microscopy
We describe experimental results on label free imaging of striated skeletal muscle using second harmonic generation (SHG) and coherent anti-Stokes Raman scattering (CARS) microscopy. The complementarity of the SHG and CARS data makes it possible to clearly identify the main sarcomere sub-structures such as actin, myosin, acto-myosin, and the intact T-tubular system as it emanates from the sarco...
متن کاملTensor-based Cortical Morphometry via Weighted Spherical Harmonic Representation
We present a new tensor-based morphometric framework that quantifies cortical shape variations using the local area element. The local area element is obtained from the Riemannian metric tensors, which are, in turn, obtained from the smooth functional parametrization of a triangle mesh. For the smooth parametrization, we have developed a novel weighted spherical harmonic (SPHARM) representation...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: IEEE Transactions on Biomedical Engineering
سال: 2012
ISSN: 0018-9294,1558-2531
DOI: 10.1109/tbme.2011.2167325